How to use the calculator
- Enter the mass of your ssRNA sample in grams (g) in the "Mass (g)" field.
- Enter the molecular weight of your ssRNA sample in g/mol in the "Molecular Weight (g/mol)" field. If you're working with a standard ssRNA sample composed of A, U, G, and C nucleotides, you can use the default value of approximately 330 g/mol.
- Click the "Calculate" button to convert the mass to moles.
- The calculator will display the number of moles and the molecular weight used in the calculation.
- If you need to start over, click the "Reset" button to clear all input fields.
Working Principle
This calculator uses the basic stoichiometric relationship between mass, molecular weight, and moles:
Moles = Mass (g) / Molecular Weight (g/mol)
This relationship allows you to convert between the mass of a substance and the number of moles when you know the molecular weight of the substance.
Example
Suppose you have a 0.5 g sample of ssRNA with a molecular weight of 330 g/mol. Using this calculator:
- Enter 0.5 in the "Mass (g)" field
- Enter 330 in the "Molecular Weight (g/mol)" field
- The calculator will display 0.001515 moles of ssRNA
Applications
This calculator is useful in molecular biology and biochemistry for:
- Preparing RNA solutions of specific concentrations
- Calculating the amount of RNA needed for experiments
- Determining the yield of RNA synthesis reactions
- Converting between different units of RNA quantification