PCR cycle calculator

PCR cycle calculator calculator can be used to calculate the total time required for a given number of PCR cycles.

Input Parameters

Number of Cycles

Denaturation Time (seconds)

Annealing Time (seconds)

Extension Time (seconds)

Calculation Results

Calculation Formula

Total Time = (Number of Cycles * (Denaturation Time + Annealing Time + Extension Time))

Where:
- Number of Cycles: Total number of PCR cycles to be performed.
- Denaturation Time: Time taken for denaturation step (in seconds).
- Annealing Time: Time taken for annealing step (in seconds).
- Extension Time: Time taken for extension step (in seconds).

Total Time (seconds)

PCR cycle calculator Calculator Usage Guide

Learn how to use the PCR cycle calculator calculator and its working principles

How to Use the PCR Cycle Calculator

Enter the number of PCR cycles you plan to perform.
Input the time (in seconds) for the denaturation step.
Input the time (in seconds) for the annealing step.
Input the time (in seconds) for the extension step.
Click the "Calculate" button to compute the total time required for all cycles.
The total time will be displayed in seconds.

Working Principle

The PCR cycle calculator calculates the total time required for a given number of PCR cycles by summing the time taken for each step (denaturation, annealing, and extension) for each cycle. The first cycle's denaturation, annealing, and extension times are included in the total time, while subsequent cycles only include the denaturation, annealing, and extension times.