Dilution Factor Calculator Dilutant to Stock Ratio

How to Use This Calculator

1. Enter the volume of your stock solution (concentrated solution) in milliliters (mL).
2. Enter the desired final concentration you want to achieve after dilution in mg/mL.
3. If you know how much dilutant (usually water or buffer) you'll be adding, enter that volume in mL. If you're not sure, leave this field blank.
4. Click the "Calculate" button to determine the dilution factor and required dilutant volume.
5. The calculator will also show you the total final volume of your diluted solution.

Understanding Dilution Factor

The dilution factor is a measure of how much a solution has been diluted. It's calculated as the ratio of the final volume to the initial volume of the solution. A higher dilution factor means a more diluted solution.

The formula used in this calculator is:

Dilution Factor = (Stock Volume × Desired Concentration) / Dilutant Volume

Applications

This calculator is useful in various laboratory applications including:

• Preparation of serial dilutions in microbiology
• Creation of standard solutions in analytical chemistry
• Adjustment of medication concentrations in pharmacy
• Preparation of working solutions from stock solutions

Example Scenario

Suppose you have 50 mL of a 1000 mg/mL stock solution and you want to prepare 500 mL of a 100 mg/mL working solution. Using the calculator:

1. Stock Volume = 50 mL
2. Desired Concentration = 100 mg/mL
3. Dilutant Volume = (50 × 1000) / 100 - 50 = 450 mL
4. Total Volume = 500 mL
5. Dilution Factor = 5